Sample solution
| Column | Welch Ultisil®XB-C18（4.6×150mm，5μm） |
| Detector | 260nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 μL |
Mobile Phase
| Time | A: Phosphate buffer(%) | Methanol(%) |
| 0 | 60 | 40 |
| 2.5 | 60 | 40 |
| 12 | 30 | 70 |
| 25 | 30 | 70 |
| 26 | 60 | 40 |
| 30 | 60 | 40 |
If you have any problem or require further information, please contact info@welchmat.com.
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