Test sample

ColumnWelch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
Mobile PhaseMobile phase A: 10mmol/L potassium dihydrogen phosphate(pH 2.9) Mobile phase B: acetonitrile
Detector215nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume50ul

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