Sample
| Column | Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043 |
| Detection | 230 nm |
| Temperature | 35℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 10 µl |
Mobile Phase
Gradient Method
| Time (min) | A (%) | B (%) |
| 0 | 100 | 0 |
| 10 | 0 | 100 |
| 15 | 0 | 100 |
| 16 | 100 | 0 |
| 20 | 100 | 0 |
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