System suitability
Gradient Program
| Time(min) | A% | B% |
| 0 | 100 | 0 |
| 15 | 0 | 100 |
| 23 | 0 | 100 |
| 24 | 100 | 0 |
| 30 | 100 | 0 |
| Column | Xtimate® C18, 4.6×150 mm, 5 μm (P/N: 00101-21041) |
| Mobile Phase | Phosphate buffer: 3.4 mL phosphate, 950 mL water, 4.3 mL 50% sodium hydroxide, add water to 1000mL and adjust pH to 7.10 with phosphoric acid or 50% sodium hydroxide solution. Mobile Phase A: Phosphate buffer/acetonitrile=98/2 Mobile phase B: Phosphate buffer/acetonitrile=78/22 |
| Detection | 280 nm |
| Temperature | 35℃ |
| Flow Rate | 1.3 mL/min |
| Injection Volume | 10 µL |
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