Reference Substance of Ranitidine

Gradient Program

Time(min)A%B%
01000
150100
230100
241000
301000
ColumnWelch Xtimate C18 4.6x150mm,5μm  (P/N: 00101-21041)
  Mobile PhaseA: Phosphate buffer (take 6.8 ml phosphoric acid in 1900 ml water, add 50% sodium hydroxide to adjust the pH to 7.1 ± 0.05) – acetonitrile = 98:2 B: phosphate buffer – acetonitrile = 78:22
Detector230nm
Temperature35℃
Flow Rate1.5ml/min
Injection Volume10ul

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