Reference Substance of Ranitidine
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 15 | 0 | 100 |
| 23 | 0 | 100 |
| 24 | 100 | 0 |
| 30 | 100 | 0 |
| Column | Welch Xtimate C18 4.6x150mm,5μm (P/N: 00101-21041) |
| Mobile Phase | A: Phosphate buffer (take 6.8 ml phosphoric acid in 1900 ml water, add 50% sodium hydroxide to adjust the pH to 7.1 ± 0.05) – acetonitrile = 98:2 B: phosphate buffer – acetonitrile = 78:22 |
| Detector | 230nm |
| Temperature | 35℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 10ul |
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