Content determination of test sample

ColumnWelch Ultisil XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
Mobile PhaseMethanol-0.1% triethylamine solution (adjust PH to 2.5 with phosphoric acid ) = 45:55
Detector230nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul

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