Endotoxin is a common protein pollutant whose existence makes the protein activity research become very complex. Besides, it is harmful to human health for it may cause fever, microcirculation disorders, endotoxin shock and disseminated endovascular coagulation and a series of adverse symptoms. Therefore, the detection and removal of endotoxins in the protein is of great significance.
Endotoxin Rem Tanrose 4FF takes self-made agarose gel as the matrix and polystatin B as the ligand group, which is used to remove endotoxins from biogenic protein products (including polypeptide, antibody, polysaccharide, etc. ). However, polystatin B only has an inhibitory effect on part of endotoxins.
Technical parameters
| Media | Endotoxin Rem Tanrose 4FF |
| Bead structure | 4% highly crosslinked agarose |
| Bead size range | 45-165 µm |
| Mean bead size | 90 µm |
| Matrix | Endotoxin substrate analogue |
| Shape | Spherical |
| Matrix density | 5 mg/ml (media) |
| Binding capacity | 5000-10000 Eu/ml (media) |
| pH stability | 3-10 (long term) 2-13 (short term) |
| Operating pressure | ≤0.3 MPa |
| Max. flow rate (25℃) | 300 cm/h |
| Ideal flow rate | 100 cm/h |
| Chemical stability | 30% isopropyl alcohol, 8.0M urea, 6.0M guanidine hydrochloride |
| Storage solution | 20% ethanol |
| Storage temperature | 4-8℃ |
What should we do about low removing efficiency?
Possible reason: The sample pH is beyond the binding range of endotoxin
Solution: Adjust the pH to 7 – 8 with the 0.1M NaOH or 0.1M HCl
Possible reason: Short contact time between the sample and the packing materials
Solution: Reduce the flow speed and increase the contact time
Possible reason: The detection system is contaminated with the endotoxin
Solution: Ensure that all test supplies are products without heat source
Possible reason: Strong binding capacity between endotoxin and target protein
Solution: Optimize the sample pH to separate sample from the endotoxin
What should we do about sample contamination?
Possible reason: The packing materials were used to purifying other samples
Solution: Increase contact time; do not use used packing materials to remove endotoxins
What should we do about low recovery rate of sample?
Possible reason: The sample is not specifically adsorbed on the packing materials
Solution: Increase the NaCl concentration in the sample and equilibrium liquid
Possible reason: Target proteins were removed combined with the endotoxin
Solution: Optimize the sample pH to separate sample from the endotoxin
Ordering Information
| Part Number | Specification |
| AE4010 | 25 mL |
| AE4011 | 100 mL |
| AE4012 | 500 mL |
| AE4013 | 1 L |
If you have any problem or require further information, please contact info@welchmat.com.
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