Endotoxin is a common protein pollutant whose existence makes the protein activity research become very complex. Besides, it is harmful to human health for it may cause fever, microcirculation disorders, endotoxin shock and disseminated endovascular coagulation and a series of adverse symptoms. Therefore, the detection and removal of endotoxins in the protein is of great significance.

Endotoxin Rem Tanrose 4FF takes self-made agarose gel as the matrix and polystatin B as the ligand group, which is used to remove endotoxins from biogenic protein products (including polypeptide, antibody, polysaccharide, etc. ). However, polystatin B only has an inhibitory effect on part of endotoxins.

Technical parameters

MediaEndotoxin Rem Tanrose 4FF
Bead structure4% highly crosslinked agarose
Bead size range45-165 µm
Mean bead size90 µm
MatrixEndotoxin substrate analogue
Matrix density5 mg/ml (media)
Binding capacity5000-10000 Eu/ml (media)
pH stability3-10 (long term) 2-13 (short term)
Operating pressure≤0.3 MPa
Max. flow rate (25℃)300 cm/h
Ideal flow rate100 cm/h
Chemical stability30% isopropyl alcohol, 8.0M urea, 6.0M guanidine hydrochloride
Storage solution20% ethanol
Storage temperature4-8℃

What should we do about low removing efficiency?

Possible reason: The sample pH is beyond the binding range of endotoxin

Solution: Adjust the pH to 7 – 8 with the 0.1M NaOH or 0.1M HCl

Possible reason: Short contact time between the sample and the packing materials

Solution: Reduce the flow speed and increase the contact time

Possible reason: The detection system is contaminated with the endotoxin

Solution: Ensure that all test supplies are products without heat source

Possible reason: Strong binding capacity between endotoxin and target protein

Solution: Optimize the sample pH to separate sample from the endotoxin

What should we do about sample contamination?

Possible reason: The packing materials were used to purifying other samples

Solution: Increase contact time; do not use used packing materials to remove endotoxins

What should we do about low recovery rate of sample?

Possible reason: The sample is not specifically adsorbed on the packing materials

Solution: Increase the NaCl concentration in the sample and equilibrium liquid

Possible reason: Target proteins were removed combined with the endotoxin

Solution: Optimize the sample pH to separate sample from the endotoxin

Ordering Information

Part NumberSpecification
AE401025 mL
AE4011100 mL
AE4012500 mL
AE40131 L

If you have any problem or require further information, please contact info@welchmat.com.

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