Impurity a1
| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Detector | 264nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20µl |
Mobile Phase
A: 0.05 mol/L dipotassium hydrogen phosphate (containing 0.4% triethylamine, adjust pH to 6.0 with phosphoric acid);
B: methanol;
| Time (min) | A (%) | B (%) |
| 0 | 72 | 28 |
| 20 | 55 | 45 |
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