First injection of newly configured samples:

ColumnUltisil® LP-C18, 5μm, 4.6×250mm    PN: 00208-31043
Mobile PhaseMix 500mL water, 40mL acetonitrile and 0.6g phosphoric acid
Detection230 nm
Temperature30℃
Flow Rate1 ml/min
Injection Volume20 µl
NotesThe impurity peak at 4.1min increased as time passed, which was due to sample degradation

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