First injection of newly configured samples:
Column | Ultisil® LP-C18, 5μm, 4.6×250mm PN: 00208-31043 |
Mobile Phase | Mix 500mL water, 40mL acetonitrile and 0.6g phosphoric acid |
Detection | 230 nm |
Temperature | 30℃ |
Flow Rate | 1 ml/min |
Injection Volume | 20 µl |
Notes | The impurity peak at 4.1min increased as time passed, which was due to sample degradation |
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