First injection of sample

Gradient Program

Time(min)A%B%
01387
101783
507030
601000
ColumnWelch Ultisil XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
Mobile PhaseA: acetonitrile-methanol (9:1), B: 20 mmol/L ammonium acetate solution (adjust pH to 3.5 with ice Acetic acid )
Detector360 nm
Temperature30℃
Flow Rate0.8ml/min
Injection Volume10ul

If you have any problem or require further information, please contact info@welchmat.com.


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