First injection of sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 13 | 87 |
| 10 | 17 | 83 |
| 50 | 70 | 30 |
| 60 | 100 | 0 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: acetonitrile-methanol (9:1), B: 20 mmol/L ammonium acetate solution (adjust pH to 3.5 with ice Acetic acid ) |
| Detector | 360 nm |
| Temperature | 30℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
If you have any problem or require further information, please contact info@welchmat.com.
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