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| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Mobile Phase | Acetonitrile: Buffer (1.0 ml of trifluoroacetic acid in 400 ml of water) = 67:33 |
| Detector | 270 nm |
| Temperature | 30°C |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 20µl |
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