Test sample solution of related substance
Gradient Program
| Time（min） | A% | B% |
| 0-20 | 50 | 50 |
| 20-27 | 50→20 | 50→80 |
| 27-45 | 20 | 80 |
| 45-50 | 50 | 50 |
| Column | Welch Ultisil XB-C18 4.6×150mm，5μm (P/N:00201-31041) |
| Mobile Phase | A：Water B: Acetonitrile |
| Detector | 241 nm |
| Temperature | 30℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
If you have any problem or require further information, please contact info@welchmat.com.
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