Blank Solution
Mobile Phase
| Time | 40mm DAP/ Methanol=80:20 | Acetonitrile: Water=90/10 |
| 0 | 98 | 2 |
| 5 | 98 | 2 |
| 30 | 50 | 50 |
| 31 | 50 | 2 |
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Detection | 210 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 10 µl |
| Specification |
Pregabalin impurity 1 impurity 2 impurity 3 impurity 4
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