Blank Solution
Mobile Phase
Time | 40mm DAP/ Methanol=80:20 | Acetonitrile: Water=90/10 |
0 | 98 | 2 |
5 | 98 | 2 |
30 | 50 | 50 |
31 | 50 | 2 |
Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
Detection | 210 nm |
Temperature | 30 ℃ |
Flow Rate | 1 ml/min |
Injection Volume | 10 µl |
Specification |

Pregabalin impurity 1 impurity 2 impurity 3 impurity 4
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