| Column | Ultisil AQ-C18, 4.6×250mm, 5μm (P/N 00207-31043) |
| Detection | 254nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time/min | Water | Acetonitril:Methanol = 1:1 |
| 0 | 60 | 40 |
| 14 | 60 | 40 |
| 20 | 20 | 80 |
| 25 | 20 | 80 |
| 26 | 60 | 40 |
| 30 | 60 | 40 |
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