Blank solution：
| Column | Welch Ultisil LP-C18 4.6×75mm，3μm |
| Detector | 238 nm |
| Temperature | 30°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10µl |
Mobile Phase
Mobile phase A：Water-phosphate buffer-acetonitrile（52:30:18）
Mobile phase B：Water-phosphate buffer-acetonitrile（10:30:60）
| Time（min） | Mobile Phase A（%） | Mobile Phase B（%） |
| 0 | 100 | 0 |
| 3.0 | 100 | 0 |
| 26.5 | 0 | 100 |
| 26.6 | 100 | 0 |
| 30 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
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