System Suitability
| Column | Ultisil Polar RP, 5μm, 4.6×250mm (P/N 00215-31043) |
| Mobile Phase | Acetonitrile : 0.1% trichloroacetic acid (adjust pH to 2.1 with phosphoric acid)=21.5:78.5 |
| Detection | 349nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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