Ceramide location map
1.1 Ultraviolet detection
| Column | Ultisil® LP-C18, 5μm, 4.6×250mm P/N: 00208-31043 |
| Detection | 210 nm, drift tube 110℃,flow rate of carrier gas 3.2L/min |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
| ultraviolet detector and Evaporative Light Scattering Detector were used in series |
Mobile Phase
| Time | A% | B% |
| 0-20 | 90-55 | 10-45 |
| 20-30 | 55-0 | 45-100 |
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