Related substance of test sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 70 | 30 |
| 20 | 0 | 100 |
| 35 | 0 | 100 |
| 50 | 70 | 30 |
| Column | Welch Ultisil XB-ALK 4.6×250mm，5μm (P/N: 00253-31043) |
| Mobile Phase | A: 0.5 mol/L potassium dihydrogen phosphate (adjust pH to 3.5 with phosphoric acid ) – methanol – Water = 10:30:60 B: 0.5 mol/L potassium dihydrogen phosphate (adjust pH to 3.5 with phosphoric acid ) – methanol – Water = 10:50:40 |
| Detector | 225nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
| Notes | Sensitive to injection volume and dissolved sample solvent |
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