Related substance of test sample

Gradient Program

Time(min)A%B%
07030
200100
350100
507030
ColumnWelch Ultisil XB-ALK 4.6×250mm,5μm (P/N: 00253-31043)
 Mobile PhaseA: 0.5 mol/L potassium dihydrogen phosphate (adjust pH to 3.5 with phosphoric acid ) – methanol – Water = 10:30:60 B: 0.5 mol/L potassium dihydrogen phosphate (adjust pH to 3.5 with phosphoric acid ) – methanol – Water = 10:50:40
Detector225nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul
NotesSensitive to injection volume and dissolved sample solvent

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