Sample

ColumnWelch Ultisil PFP 4.6×150mm,3μm (P/N: 00224-21041)
Detector215nm
Temperature40℃
Flow Rate1.0 ml/min
Injection Volume10µl

Mobile Phase

0.1% TFA water/methanol

Time(min)0.1% TFA water(%)Methanol(%)
04555
204555
401090

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