Sample
Column | Welch Ultisil PFP 4.6×150mm,3μm (P/N: 00224-21041) |
Detector | 215nm |
Temperature | 40℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 10µl |
Mobile Phase
0.1% TFA water/methanol
Time(min) | 0.1% TFA water(%) | Methanol(%) |
0 | 45 | 55 |
20 | 45 | 55 |
40 | 10 | 90 |
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