Gradient Condition

Time(min)A%B%
04555
204555
401090
ColumnWelch Ultisil® PFP, 3 um, 4.6×150 mm
Mobile PhaseMobile Phase: A
Mix 0.5 mL trifluoroacetic acid with 500 ml water of and filter it
B: Pure methanol
Wavelength215 nm
Temperature40℃
Flow Rate1.0 mL/min
Injection Volume10 μL

If you have any problem or require further information, please contact info@welchmat.com.


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