Gradient Condition
| Time（min） | A% | B% |
| 0 | 45 | 55 |
| 20 | 45 | 55 |
| 40 | 10 | 90 |
| Column | Welch Ultisil® PFP, 3 um, 4.6×150 mm |
| Mobile Phase | Mobile Phase: A Mix 0.5 mL trifluoroacetic acid with 500 ml water of and filter it B: Pure methanol |
| Wavelength | 215 nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 μL |
If you have any problem or require further information, please contact info@welchmat.com.
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