Sample+impurity
1.1 Full Scale
| Column | Xtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043) |
| Detection | 289 nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |
Mobile Phase
| Time | 10mmol/L dipotassium hydrogen phosphate (pH7.0) | Acetonitrile |
| 0 | 90 | 10 |
| 30 | 60 | 40 |
| 45 | 15 | 85 |
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