1.Sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 81 | 19 |
| 2 | 81 | 19 |
| 8 | 60 | 40 |
| 9 | 5 | 95 |
| 10 | 5 | 95 |
| 10.01 | 81 | 19 |
| 11 | 81 | 19 |
| Column | Welch Ultisil UHPLC Polar RP 2.1×50mm，1.8μm (P/N: 00215-11010) |
| Mobile Phase | A: Water B: Acetonitrile |
| Detector | 203nm |
| Temperature | 25℃ |
| Flow Rate | 0.6ml/min |
| Injection Volume | 2ul |
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