Determination of Related Substances in Amoxicillin and Clavulanate Potassium Granules by Xtiamte C18

Column Xtimate® C18(4.6×150mm,5μm) Mobile Phase Mobile phase A: 0.01 mol/L Potassium Dihydrogen Phosphate Solution (pH 6.0 adjusted with 2 mol/L Sodium Hydroxide Solution). Mobile phase B: 0.01mol/L potassium dihydrogen phosphate solution (adjusted with 2mol/L sodium hydroxide solution section pH6.0)/acetonitrile=20/80 Detection 230nm Temperature 35℃ Flow Rate 1.0ml/min Injection Volume 20 μL

The problem of baseline drift, have you solved it?

Baseline drift can be a real headache for researchers and analysts working with data. It refers to the gradual and undesirable changes in the baseline signal of a measurement, which can obscure or distort the true variations in the data over time. Common causes of baseline drift include changes in temperature, pressure, humidity, or instrument performance, as well as sample evaporation or degradation. Here we have listed some common problems of baseline drift for you.  Read more…

The Hidden “Killer” of Chromatographic Peak Shape – Solvent Effect.

Have you ever encountered issues such as fronting, peak broadening, splitting, etc. during liquid phase system analysis? Why does reducing the injection amount of the same sample sometimes result in a better-looking chromatographic peak? In daily analysis work, we usually focus more on the selection of mobile phase and instrument methods, and neglect the importance of sample injection solvents. If the solvent selection is improper, it will lead to “solvent effects”, causing errors in qualitative Read more…

Do you really know SPE cartridge? And how to choose them?

Solid-phase extraction, abbreviated as SPE, is a sample pre-treatment device developed from chromatographic columns for extraction, separation, and concentration. It is developed by combining liquid-solid extraction cartridges with liquid chromatography technology and is mainly used for sample separation, purification, and concentration. Compared with traditional liquid-liquid extraction methods, SPE can improve the recovery rate of analytes, more effectively separate analytes from interfering components, reduce sample preparation processes, and is simple, time-saving, and labor-saving to operate. It Read more…

How to choose the right HPLC Vials?

For chemical experiments, all results are typically stage-dependent, which involves issues of sample storage and sampling, as well as how to choose appropriate HPLC Vials based on the characteristics of the samples themselves to minimize experimental errors and save costs. Sample Vials include HPLC vials, headspace vials, storage vials, TOC vials, etc. This time, we mainly introduce the selection method of injection vials. 01 SEALING METHODS FOR SAMPLE VIALS Screw thread Cap: a sealing method that Read more…

2023 Welch Materials Exhibit Info

We are thrilled to announce that Welch Materials will be attending the following exhibition! This is an exciting opportunity for us to showcase our products and services to a wider audience. At our booth, you will have the opportunity to learn more about us and what we have to offer. Our team of experts will be on hand to answer any questions you may have and to help you find the products that best meet Read more…

Ultisil LP-C18 Separate FP and its chiral isomers

Column Ultisil LP-C18 Separate FP and its chiral isomers Mobile Phase Mobile phase A: 0.01mol/L sodium dihydrogen phosphate solution (containing 10g/L sulfonyl-β-cyclodextrin sodium salt, adjust the pH value to 2.0 with phosphoric acid Time Mobile Phase A Methanol (%) 0-20 75-65 25-35 20.1-25 75 25 Detection 205nm Temperature 30℃ Flow Rate 0.8ml/min Injection Volume 10 μL (high concentration standard mix); 20 μL (low concentration standard mix)

Determination of Sitagliptin metformin by Ultisil® ODS-3

Column Ultisil® ODS-3(4.6×150mm,3.5μm) Mobile Phase Mobile phase A: Take 3.9g of sodium dihydrogen phosphate dihydrate, add it to 1000mL of water, mix well, and adjust the pH to 2.5 with phosphoric acid; take 950mL of the above solution, mix it with 50mL of acetonitrile and shake well, filter it with suction, and degas it by ultrasonic. Detection 205nm Temperature 40℃ Flow Rate 1.0 ml/min Injection Volume 20 μL