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Gradient Program
| Time（min） | Acetonitrile（%） | Water（%） |
| 0 | 40 | 60 |
| 45 | 40 | 60 |
| 70 | 80 | 20 |
| 80 | 40 | 60 |
| 90 | 40 | 60 |
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Detector | 227 nm |
| Temperature | 30℃ |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 10µl |
| Notes |
If you have any problem or require further information, please contact info@welchmat.com.
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