Mixed Reference, 5ul

Without suppressor

ColumnUltisil XB-C18, 5μm, 4.6×300mm (P/N 00201-31044)
Mobile PhaseAcetonitrile : Triethanolamine Phosphate Buffer(pH7.7) = 5:95
Detection280nm
Temperature30℃
Flow Rate2.0ml/min
Injection Volume5ul(solvent effect); 2.5uL
NoteLeading peaks due to p-phenylenediamine; decrease injection volume or add suppressor

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