| Column | Ultisil® LP-C18, 5μm, 4.6×300mm PN: 00208-31044 |
| Mobile Phase | acetonitrile:1.4g/L disodium hydrogen phosphate adjust pH to 7.6 with phosphate=28:72 |
| Detection | 280 nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
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