1.0ml/min

ColumnUltisil XB-CN, 5μm, 4.6×250mm (P/N 00205-31043)
Mobile PhaseWater:Methanol=300:200 (containing 10g/L ammonium acetate, adjust pH to 4.0 with HCL)
Detection260nm
Temperature30℃
Flow Rate1.0ml/min or 1.5ml/min
Injection Volume20ul

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