ColumnTopsil LP-C18, 4.6×250mm, 5μm (P/N 00208-31043)
Mobile PhaseAcetonitrile:Water:Perchloric acid(pH1.69) = 55:44.95:0.05
Detection220nm
Temperature25℃
Flow Rate0.8ml/min
Injection Volume20ul
NoteAvoid water when preparing mobile phase

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