Column | Topsil LP-C18, 4.6×250mm, 5μm (P/N 00208-31043) |
Mobile Phase | Acetonitrile:Water:Perchloric acid(pH1.69) = 55:44.95:0.05 |
Detection | 220nm |
Temperature | 25℃ |
Flow Rate | 0.8ml/min |
Injection Volume | 20ul |
Note | Avoid water when preparing mobile phase |
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