Column | Ultisil® LP-C18, 5μm, 4.6×250mm PN: 00208-31043 |
Mobile Phase | Acetonitrile: water: perchloric acid =55:44.95:0.05 (perchloric acid solution pH=1.69) |
Detection | 220 nm |
Temperature | 25℃ |
Flow Rate | 0.8 ml/min |
Injection Volume | 20 µl |
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