Norfloxacin, enoxacin, ciprofloxacin(278 nm)
1.1 System suitability solution
| No. | Component | Rt (min) | Height (mAU) | Area (mAU*min) | Theoretical plate(EP) | Asymmetry (A|A) | R(EP) |
| 1 | Enoxacin | 8.423 | 23.283 | 4.319 | 14763 | 1.34 | 4.62 |
| 2 | Norfloxacin | 9.990 | 1220.180 | 322.963 | 9824 | 2.91 | 4.11 |
| 3 | Ciprofloxacin | 11.590 | 22.297 | 5.436 | 15132 | 1.32 | n.a. |
| Column | Ultisil® XS-C18（4.6×250mm，5μm） |
| Detection | 278 nm(other impurities), 262nm(impurity A) |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µL |
Mobile Phase
A: 0.025 mol/L Phosphoric acid solution(adjust pH 3.0 with triethylamine)/acetonitrile=87/13 B:acetonitrile
| Time(min) | A(%) | B(%) |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 20 | 50 | 50 |
| 30 | 50 | 50 |
| 32 | 100 | 0 |
| 42 | 100 | 0 |
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