Solution of test sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 70 | 30 |
| 2 | 70 | 30 |
| 13 | 20 | 80 |
| 25 | 20 | 80 |
| Column | Welch Ultisil XB-C18 21.2×250mm，10μm (P/N: 02610-31106) |
| Mobile Phase | A：10mmol/L ammonium bicarbonate solution B：Acetonitrile |
| Detector | 218 nm |
| Temperature | Room Temperature |
| Flow Rate | 20ml/min |
| Injection Volume | 1000ul |
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