Solution of test sample
Gradient Program
Time(min) | A% | B% |
0 | 70 | 30 |
2 | 70 | 30 |
13 | 20 | 80 |
25 | 20 | 80 |
Column | Welch Ultisil XB-C18 21.2×250mm,10μm (P/N: 02610-31106) |
Mobile Phase | A:10mmol/L ammonium bicarbonate solution B:Acetonitrile |
Detector | 218 nm |
Temperature | Room Temperature |
Flow Rate | 20ml/min |
Injection Volume | 1000ul |
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