Test Sample

ColumnWelch Xtimate C18 4.6x250mm,5μm  (P/N: 00101-21043)
Mobile Phase0.5% triethylamine solution (pH adjusted to 3.0 with phosphoric acid) – tetrahydrofuran-acetonitrile = 60:10:30
Detector280nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul
NotesSensitivity to injection volume and solvent for dissolving sample

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