Biotin reference

ColumnWelch Ultisil Polar RP 4.6×300mm,5μm (P/N: 00215-31044)
Mobile Phase0.05% trifluoroacetic acid water (pH 2.5): acetonitrile = 85:15
Detector210nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume20ul

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