Mixed sample
| Column | Xtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043) |
| Mobile Phase | (1g/L anhydrous copper sulfate, 1g/L l-isoleucine, adjust pH to 4.0 with sodium hydroxide) /methanol =66/34 |
| Detection | 293 nm |
| Temperature | 40℃ |
| Flow Rate | 1.5 mL/min |
| Injection Volume | 10 µL |
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