| Column | Xtimate C18, 3μm, 4.6×250mm PN: 00101-11043 |
| Detection | 254 nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: acetonitrile; B: 50Mmol potassium dihydrogen phosphate buffer
| Time (min) | A (%) | B (%) |
| 0 | 17 | 83 |
| 5 | 17 | 83 |
| 25 | 15 | 82 |
| 30 | 17 | 83 |
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