Sample solution
| Column | Ultisil XB-C18 4.6×150 mm, 5 μm (P/N: 00201-31043) |
| Detection | 254 nm |
| Temperature | 30°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 µl |
Mobile Phase
| Time(min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 80 | 20 |
| 1 | 80 | 20 |
| 6 | 65 | 35 |
| 25 | 2 | 98 |
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