Condition 1:

ColumnUltisil® Cellu-J, 5μm, 4.6×250mm    PN: 00218-31043
Mobile PhaseN-Hexane: Alcohol: Diethylamine =70/30/0.1 Condition 1 N-Hexane: Alcohol: Methanol: Diethylamine =70/30/1/0.1
Condition 2 N-Hexane: Alcohol: Methanol: Diethylamine =60/30/10/0.1 Condition 3
Detection251 nm
Temperature30 ℃
Flow Rate1 ml/min
Injection Volume2-10 µl
NotesMobile Phase should be intensive mixing.

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