Condition 1:
| Column | Ultisil® Cellu-J, 5μm, 4.6×250mm PN: 00218-31043 |
| Mobile Phase | N-Hexane: Alcohol: Diethylamine =70/30/0.1 Condition 1 N-Hexane: Alcohol: Methanol: Diethylamine =70/30/1/0.1 Condition 2 N-Hexane: Alcohol: Methanol: Diethylamine =60/30/10/0.1 Condition 3 |
| Detection | 251 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 2-10 µl |
| Notes | Mobile Phase should be intensive mixing. |
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