Sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 15 | 100 | 0 |
| 30 | 80 | 20 |
| 60 | 0 | 100 |
| 80 | 0 | 100 |
| 100 | 100 | 0 |
| 120 | 100 | 0 |
| Column | Welch Ultisil XB-SCX 10×250，5μm (P/N: 02614-21102) |
| Mobile Phase | Mobile phase A: 0.5 mmol/L sulfuric acid solution of 7 mmol/L sodium sulfate: acetonitrile = 40:60; Mobile phase B: 0.5 mmol/L sulfuric acid solution of 56 mmol/L sodium sulfate: acetonitrile = 40:60; |
| Detector | 250nm |
| Temperature | 45℃ |
| Flow Rate | 3ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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