System suitability solution
| Column | Xtimate® C18, 4.0×150mm, 3μm (P/N: 00101-11032) |
| Detection | 275 nm |
| Temperature | 37℃ |
| Flow Rate | 0.9 mL/min |
| Injection Volume | 5 µL |
| Notes | A Ghost-Buster column should be installed in front of the sampler to remove mobile phase interference |
Mobile Phase
Mobile phase A: 5g/L ammonium acetate solution, adjust pH 7.0 with ammonia water;
Mobile Phase B: acetonitrile
| Time | Mobile Phase A(%) | Mobile Phase B(%) |
| 0 | 95 | 5 |
| 1.5 | 95 | 5 |
| 16.5 | 42.5 | 57.5 |
| 21.5 | 95 | 5 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments