System Suitability

ColumnUltisil Polar RP, 5μm, 4.6×250mm (P/N 00215-31043)
Mobile PhaseA: Acetonitrile:23g/L phosphoric acid:water=0.5:12.5:87 B: 23g/L phosphoric acid:water:acetonitrile=12.5:40:47.5 Gradient
Detection205nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume50ul
NoteUse Ghost-Buster column for ghost peaks

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