Reference

ColumnWelchrom® C18, 4.6×250mm, 5μm (P/N: 00310-02043)
Mobile Phase0.0025 M sodium octanesulfonate solution /[methanol: acetonitrile (3:2)]=60:40 (adjust pH 3.0 with 1% phosphoric acid)
DetectionUV: Related substance 254 nm, assay 266 nm
Temperature30℃
Flow Rate1.0 mL/min
Injection Volume20 µL

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