Sample
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Mobile Phase | Buffer salt (potassium dihydrogen phosphate 1.1g, sodium octane sulfonate 1.78g; placed in 720ml water,adjust pH to phosphoric acid to 2.2)-methanol-acetonitrile = (720:180:100) |
| Detector | 220 nm |
| Temperature | 25°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20µl |
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