Sample

ColumnWelch Ultisil LP-C18 4.6×250mm,5μm (P/N: 00208-31043)
Mobile PhaseBuffer salt (potassium dihydrogen phosphate 1.1g, sodium octane sulfonate 1.78g; placed in 720ml water,adjust pH to phosphoric acid to 2.2)-methanol-acetonitrile = (720:180:100)
Detector220 nm
Temperature25°C
Flow Rate1.0 ml/min
Injection Volume20µl

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