1. Sample

ColumnWelch Ultisil®LP-C8(4.6*250mm,5μm)
 Mobile PhaseBuffer salt (take 1.1g potassium dihydrogen phosphate, 1.78g sodium octane sulfonate in 720ml water. Adjust pH to 2.2 with phosphoric acid) -methanol-acetonitrile = (720:180:100)
WavelengthRefractive index detector Detector temperature: 40℃
Flow Rate0.5 mL/min
Injection Volume20 μl

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