Column | Ultisil LP-C18, 4.6×250mm, 5μm (P/N 00209-31043) |
Detection | 214nm |
Temperature | 30℃ |
Flow Rate | 1.0ml/min |
Mobile Phase
Time/min | Methanol | Water |
0 | 90 | 10 |
8 | 90 | 10 |
14 | 65 | 35 |
15 | 90 | 10 |
25 | 90 | 10 |
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