Ultisil XB-C8（4.6X250mm，5μm，P/N：00202-31043）
1.1 Cis acid
| Column | Welch Ultisil XB-C8，4.6x250mm，5μm（P/N：00202-31043) Welch Welchrom C8，4.6x250mm，5μm（P/N：00320-02043) |
| Mobile Phase | Phosphoric acid / methanol / water = 1 / 100 / 900 |
| Detector | 214nm |
| Temperature | 20℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 5ul |
| Notes | The impurity peak between cis acid and succinic acid is sensitive to temperature |
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