Mixed standard solution
Gradient Program
| Time（min） | A% | B% |
| 0 | 95 | 5 |
| 10 | 70 | 30 |
| 15 | 60 | 40 |
| 25 | 60 | 40 |
| 26 | 95 | 5 |
| 35 | 95 | 5 |
| Column | Welch Ultisil Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | A:25mmol/L ammonium dihydrogen phosphate (pH7.0) B:Methanol |
| Detector | 280nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 5ul |
If you have any problem or require further information, please contact info@welchmat.com.
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