Solution of β-carotene
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm |
| Mobile Phase | Mobile phase A : methanol; mobile phase B: acetonitrile / tetrahydrofuran = 60/40; Mobile phase A / mobile phase B = 55/45; two phases mixed in line |
| Detector | 260nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 10µl |
| Notes | Lycopene has a solvent effect at 20 μl injection volume |
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