Sample Solution
Column | Ultisil ALK C18, 5μm, 4.6×250mm (P/N 00253-31043) |
Mobile Phase | Phosphate buffer(0.02mol/L NaH2PO4, adjust pH to 5 with NaOH solution):Acetonitrile=92:8 |
Detection | 215nm |
Temperature | 50℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
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