Phthalates, also known as phthalates, abbreviated PAEs, are the general term for esters formed by phthalic acid, and are a kind of plasticizer. When used as plastic plasticizers, it generally refers to the Esters of phthalic acid and alcohols of 4 to 15 carbons, of which dioctyl phthalate is the most important variety, and is a class of chemicals that can soften the endocrine system.

On October 27, 2017, the list of carcinogens published by the World Health Organization’s International Agency for Research on Cancer was preliminarily sorted for reference, and bis(2-ethylhexyl) phthalate was included in the list of class 2B carcinogens.

Chromatographic conditions

Chromatographic column: Welch Ultisil® LP-C18 (4.6×300mm, 5μm).

Time(min)Methanol (%)Water(%)
07525
127525
151000
221000
237525
257525
307525

Detection wavelength: 280nm;
Column temperature: 35℃;
Flow rate: 1.0mL/min;
Injection volume: 5 μL.

Chromatogram and data

Mixed standard solution
P/NCompoundRTHeightAreaPlate noTailingSeparation
1DMP4.999142527775473150661.170
2DEP6.60098143663519184561.0908.962
3DPP10.41361617624271224661.02016.150
4BBP17.97530778479433295360.93621.721
5DBP18.76250166519932693640.9462.237
6DAP21.1501182684414865547831.12611.986
7DCHP21.385103432417843500709
1.110
1.998
8DHP22.180935404072074680411.0976.351
9DEHP23.94158896356289
296007

1.024
11.521
10DOP24.490548823553112706841.0153.012
Total8122505039763

Conclusion

Using Welch Ultisil® LP-C18 (4.6×300mm, 5μm) to measure under these chromatographic conditions, it can meet the detection requirements.


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