Gradient Program
| Time | A:0.1% TFA aqueous solution | B:0.1%TFA Acetonitrile solution |
| 0 | 90 | 10 |
| 15 | 70 | 30 |
| 30 | 25 | 75 |
| 35 | 25 | 75 |
| 36 | 90 | 10 |
| 45 | 90 | 10 |
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Detector | 254 nm |
| Temperature | 30°C |
| Flow Rate | 1 ml/min |
| Injection Volume | 10µl |
If you have any problem or require further information, please contact info@welchmat.com.
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